Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for a Principal Scientist, Analytical to be located in Malvern, PA.
At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at www.janssen.com and follow us @JanssenGlobal. Janssen Research & Development, LLC is part of the Janssen Pharmaceutical Companies.
The Biotherapeutics Development-Analytical Development (BioTD-AD) group is seeking an upbeat, highly motivated leader to characterize next-generation, iPSC-based CAR-T cell platforms for allogeneic cell therapies. The Principal Scientist will establish the technical strategies for generating engineered iPSC-derived g/d T-cell therapies for oncology and other indications.
The Principal Scientist will shape the strategies required for in depth process and product understanding and provide technical and strategic leadership to the function and to the Chemistry Manufacturing Control (CMC) teams. The individual will have a strong foundation in human T cell and stem cell biology. Experience with the design, execution and optimization of a broad range of in vitro cell based characterization assays, and will have the opportunity to engage in multi-functional teams. They will learn from and contribute to industry-leading drug discovery/development programs that has brought numerous quality therapeutics to patients and has made significant contributions to human health.
- Develop characterization strategies and implements plans to characterize iPSCs, cell and gene engineering, and cell differentiation, in particular gamma/delta T cells, NK cells or related cell types.
- Lead a team of scientists developing and optimizing analysis methods that fully characterize human iPSCs and iPSC-derived cells using multi-parameter flow cytometry and in vitro assays for cell proliferation, cytokine secretion, polyfunctionality, and target cell killing.
- Manage characterization plans for assessing on-target and off-target editing activity by ICE, flow cytometry, ELISA, qPCR, western blot, and others.
- Direct technical forums both internal and external to the company to share knowledge and approaches related to cellular characterization strategies as they relate to cell-based therapies (CAR-T, CAR-NK, etc.).
- Engage and/or lead technical investigation strategies in support of product investigations with respect to clinical outcomes or adverse events driving to root cause.
- Develop innovative approaches to challenging solutions.
- Identify/collaborate with partners in academia or industry to implement innovation ideas or characterization approaches.
- Effectively communicate scientific data and results across technical teams, project teams, and to the health authority as needed.
- M.S. degree with 10 years of experience in an academic or industry setting with a focus in the field of human iPSC biology or
- Ph.D. in cell biology, immunology, biological science, or related field with 7 years of experience.
Experience and Skills:
- Proven experience in NK/T cell biology, function and iPSC-NK/T cell differentiation is required.
- Human iPSC subject matter expert able to apply skills, theoretical knowledge, and experience to provide scientific direction and approach to projects and challenges is required.
- Ability to work collaboratively in a complex, environment is required.
- Expertise with primary T-cell and pluripotent SC culture, viral and non-viral gene delivery methods and cellular based assays is required.
- Experience with in vitro technologies to assess lymphocyte functionality including but not limited to multi-parameter flow cytometry, MSD, Luminex, xCELLigence and IncuCyte-based assays, for phenotypic characterization of T/NK cells, T/NK cell activation, cytotoxicity and proliferation assays is required.
- Excellent oral and written communication skills is required.
- Ability to independently design, implement and troubleshoot experiments, analyze, reports and present data is required.
- Must be detailed, highly organized, able to manage multiple tasks with tight timelines and deliver quality data.
- Knowledge of the connections between clinical, process/product development, and biological outcomes is preferred.
- Experience with the development and registration of cell therapies is a plus.
- Awareness of GMP requirements is desired.
- Ability to make timely decisions and operate effectively in times of ambiguity is highly preferred.
- Strong analytical problem solving, planning and organizational skill is desired.
- People leadership experience, working in a team or direct line setting, is a plus.
At Johnson & Johnson, we’re on a mission to change the trajectory of health for humanity. That starts by creating the world’s healthiest workforce. Through cutting-edge programs and policies, we empower the physical, mental, emotional and financial health of our employees and the ones they love. As such, candidates offered employment must show proof of COVID-19 vaccination or secure an approved accommodation prior to the commencement of employment to support the well-being of our employees, their families and the communities in which we live and work.
Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.
For more information on how we support the whole health of our employees throughout their wellness, career and life journey, please visit www.careers.jnj.com