Johnson & Johnson Careers

Senior Scientist, Reagent Generation

Spring House, Pennsylvania

Job Description

Requisition ID: 9989190321

Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for a Senior Scientist located in Spring House, PA.

At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at and follow us @JanssenGlobal.  Janssen Research & Development, LLC is part of the Janssen Pharmaceutical Companies.

This is a Senior Scientist level position within the Reagent Generation team in the Biologics Discovery group of Janssen BioTherapeutics (JBIO). The Biologics Discovery group is responsible for discovering, characterizing and analyzing lead molecules for biotherapeutic drug development across all therapeutic areas of J&J. This position supports JBIO through the design and production of protein, cell, DNA, other reagents to enable discovery immunization campaigns and the subsequent screening of therapeutic panels. The successful candidate will possess expertise in recombinant protein production and mammalian cell engineering. The position also requires data analysis, interpretation of the results, preparation of reports and effective communication of results to management and program teams. This position may also include responsibilities for the supervision and development of associate scientists. As a Senior Scientist you will:

  • You will collaborate with project teams from all therapeutic areas to identify the most promising and appropriate designs for immunogens and screening reagents.
  • Produce and oversee the production of protein, cell, and DNA reagents to support antibody generation and screening for biotherapeutic drug discovery.
  • Provide oversight and mentorship to junior group members and external organizations.
  • Characterize the quality of protein, cell, and DNA reagents, review biophysical data from external organizations, and collaborate with other functional groups within JBIO to ensure immunogens are representative of native targets.


  • A minimum of a PhD is required

  • A minimum of 2 years of post-doctoral OR at least 2 years of industry experience is required

  • Expertise in the expression, purification, and characterization of proteins from numerous hosts including mammalian cells (HEK, CHO, NS0) is preferred

  • Expertise in mammalian cell engineering including the production of monoclonal and stable pools over-expressing a specific target of interest, as well as production of gene-edited cell lines by CRISPR-Cas9 is required

  • Expertise in flow cytometry to analyze cell surface as well as intracellular target expression is required

  • Proficient use of public databases to extract relevant data – sequences, structural domains, signal peptide analysis and design is required

  • Protein engineering experience as well as understanding of structure of antibodies and antibody-like molecules including single domain/VHH, scFv, Fab, etc is preferred

  • Advanced understanding of structural biology particularly as it pertains to the design of protein sub-domains, fusion proteins, and chimeras is preferred

  • Experience generating engineered lines from B cell lymphoma lineage is preferred

  • Experience in fluorescence activated cell sorting (FACS) preferred

  • Working understanding of immunology, physiology of immune response in different pre-clinical models preferred


Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability.

Primary Location
United States-Pennsylvania-Spring House-
Janssen Research & Development, LLC. (6084)
Job Function
Requisition ID