Johnson & Johnson Careers

Associate Scientist II, Lentivirus & CAR-T Production (1 of 2)

Spring House, Pennsylvania
R&D


Job Description

Requisition ID: 5436190125

Janssen Research & Development, L.L.C., a division of Johnson & Johnson's Family of Companies is recruiting for an Associate Scientist II, Lentivirus & CAR-T Production located in Spring House, PA.
 
At the Janssen Pharmaceutical Companies of Johnson & Johnson, what matters most is helping people live full and healthy lives. We focus on treating, curing and preventing some of the most devastating and complex diseases of our time. And we pursue the most promising science, wherever it might be found. 
 
Janssen Research & Development, LLC discovers and develops innovative medical solutions to address important unmet medical needs in oncology, immunology, neuroscience, infectious diseases and vaccines, and cardiovascular and metabolic diseases. Please visit http://www.janssenrnd.com/ for more information. 
 
We are Janssen. Our mission drives us. Our patients inspire us. We collaborate with the world for the health of everyone in it.
 
Thriving on a diverse company culture, celebrating the uniqueness of our employees and committed to inclusion. Proud to be an equal opportunity employer.
 
Janssen Biologics (JBIO), Janssen Research and Development, L.L.C., a member of Johnson and Johnson's Family of Companies, is recruiting for a Research Scientist located in Spring House, PA. Biotherapeutics works on projects from five Therapeutic Areas (TA) and enables discovery of new therapeutic concepts in collaboration with TA discovery counterparts. Supporting the CAR-T Platform group within Biotherapeutics, this position will be accountable for early discovery efforts to engineer CAR-T cells for initial functional characterization in conjunction with the Biotherapeutics team and Oncology Therapeutic Area stakeholders. The key responsibilities include, but are not limited to: daily maintenance of cell cultures to enable the production of lentiviral particles and/or CAR-T cells; working knowledge of viral-based production technologies and quality control (QC) techniques; ability to transduce primary human T-cells with both lenti- and adeno-associated viral particles; utilize flow cytometric techniques to determine transduction efficiency and to perform related QC measurements of engineered cells; provide functional and intellectual support to the CAR-T cell group within JBIO.
 
Essential Functions:
  • Strong experience in research-scale lentivirus vector/particle generation
  • Working knowledge of assays that measure infectious and physical viral titer
  • Expertise in primary T cell gene transfer via lentiviral transduction
  • Isolation of primary human immune cells, including T-lymphocytes, from human blood and subsequent aseptic cell culture
  • Phenotypically characterize genetically modified primary T-lymphocytes using flow cytometric instrumentation
  • Viral vector-based or electroporation-based modification of tumor cell lines and primary human T-cells
  • Maintain an electronic lab notebook and contribute to support documentation in line with company standards
  • Support the CAR-T R&D team in JBIO to enable timely development and delivery of novel molecular entities to therapeutic area stakeholders
  • Self-motivated and eager to take on temporary responsibilities outside of initial job description

Qualifications
  • B.Sc. with 1-3 years of T cell biology experience in academic or industrial background
  • Minimum of 2 years’ experience with lentiviral-based gene delivery (primary T-cell preferred)
  • Ability to work independently with advanced flow cytometry equipment
  • Expertise in mammalian cell culture, particularly with primary human T-cells, is essential
  • Outstanding collaborative and communication skills
  • Foster a working environment that encourages the company's commitment to equal employment opportunity and the value of a diverse work force

Primary Location
United States-Pennsylvania-Spring House-1400�McKean�Road
Organization
Janssen Research & Development, LLC. (6084)
Job Function
R&D
Requisition ID
5436190125