Johnson & Johnson Careers

Performance Matching of Early Stage USP equipment - Internship

Leiden, Netherlands

Job Description

Requisition ID: 1905735598W

Performance Matching of Early Stage USP equipment
Estimated time: Internship, 6 months – 1 year

The challenge
The Bacterial Vaccines department (BacVac) is working on the discovery and early development of novel vaccines for bacterial infectious diseases. BacVac’s Process development group has a very flexible platform that can service diverse project needs, from antigen production to early-stage manufacturability assessment. Within our activities, fermentation plays a key role and our department has different throughput scales and platforms.
To ensure reproducibility of our results, we use standardized protocols and operating conditions, which can be different between brands of fermenters, shake flasks, and micro-flasks. This leads to differences in performance across scales. The aim of this internship is to suggest improvements for our current operating conditions to ensure the reproducibility across different scales.
Deliverable: Scale up/down protocols between different platforms

 The ideal candidate
The internship is ideal for students who has enrolled in Masters in fermentation technology, biochemical engineering, microbial physiology, biotechnology, biochemistry or similar degree programs. The internship can be part of the final phase of graduation studies.
The person is expected to work in the lab (hands on) and must be proficient or demonstrate sufficient knowledge of the following concepts and techniques:
  • Fermentation Engineering with basic knowledge of mass balances, scale up and scale down
  • Microbiological techniques (BSL I)
  • Microbial physiology
  • Operation of bench top fermenters, preferably with E. coli
  • Data interpretation of fermentation profiles and in-process control analysis
  • Analytical techniques: HPLC, SDS-PAGE, Bradford, plasmid stability
  • Scientific writing and presentation skills
  • Service oriented
  • Team player
  • Eagerness to learn and share knowledge
Some desirable skills (nice to have) are:
  • Knowledge about work with BSL II organisms
  • Systems Biology
  • Modelling
  • Matlab
The Reward
The opportunity to experience cutting-edge industrial R&D within the pharma sector and contribute to enable the production of a new generation of bacterial vaccines.

Primary Location
Netherlands-South Holland-Leiden-
Janssen Vaccines & Prevention BV (8852)
Job Function
Requisition ID