Johnson & Johnson Careers

Protein Expression Scientist, Cell Line Development

Spring House, Pennsylvania

Job Description

Requisition ID: 0313190724

The Biotherapeutics Development department of Janssen R&D is seeking an ambitious Scientist to join the Cell Line Development group located in Spring House, PA. Janssen R&D discovers and develops novel solutions to support the unmet medical needs in oncology, immunology, neuroscience, infectious disease, and cardiovascular disease. Our group specifically generates cell lines that produce large molecule therapeutics in support of the fast-growing pipeline for these various therapeutic areas.

At the Janssen Pharmaceutical Companies of Johnson & Johnson, we are working to create a world without disease. Transforming lives by finding new and better ways to prevent, intercept, treat and cure disease inspires us. We bring together the best minds and pursue the most promising science. We are Janssen. We collaborate with the world for the health of everyone in it. Learn more at and follow us @JanssenGlobal. 

You would conduct laboratory experiments to support development and characterization of cell lines expressing the highest quantity and quality therapeutic proteins. Responsibilities include:

  • Developing mammalian cell lines secreting complex proteins
  • Cell culture development for all protein therapeutics
  • Genomic engineering
  • Building novel expression systems
  • Evaluating and developing new technologies in cell biology, molecular biology, or equipment to improve protein yields and shorten timelines
  • Participating in projects from Lead Optimization to New Molecular Entity declaration within a cross-functional environment

Additional responsibilities include crafting experimental design, data analysis, troubleshooting, providing thorough training and mentorship to colleagues, contributing to project teams, and maintaining detailed records. We work closely with many other teams at Janssen, so you should have excellent communication skills and want to be part of a team. This position will develop into a supervisory role and may perform other duties as assigned.

  • PhD in biological sciences, biochemistry, chemical engineering, or related scientific discipline is required.

  • A minimum of 1 year of experience in one of the following areas: CRISPR/Cas9 genome editing, Cre-Lox or FLP-FRT recombination, medium development, automation/robotics development, lentivirus or AAV production, or cell culture/process optimization is a preference.

  • Experience in mammalian cell culture is required.

  • Experience working in a matrix environment and connecting with different functional groups and people in multiple levels is preferred.

  • Excellent social, organizational, verbal and written communication skills are required.

Johnson & Johnson is an Affirmative Action and Equal Opportunity Employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, age, national origin, or protected veteran status and will not be discriminated against on the basis of disability

Primary Location
United States-Pennsylvania-Spring House-
Janssen Research & Development, LLC (6084)
Job Function
Requisition ID